The effect of in vitro stimulation of rat parotid gland with the neuropeptides substance P, calcitonin gene-related peptide and galanin has been studied by microfi lament fl uorescence staining and in semithin sections, and compared to control incubations and in vitro stimulation with b-adrenergic and muscarinic agonists. Clear-cut aspects of massive granule exocytosis and cytoplasm vacuolation, indicative of protein and fl uid secretion respectively, were obvious only after substance P stimulation, whereas treatment with galanin and calcitonin gene-related peptide produced little to no morphological changes. The results being in agreement with the outcome of other methodological approaches, these procedures appear reliable, may be effectively applied to the study of the functional regulation of secretory mechanisms, and may be particularly useful in human tissue analyses.
Morphological changes induced by neuropeptide in vitro stimulation of the rat parotid gland
SERRA, MARIA PINA;QUARTU, MARINA
2010-01-01
Abstract
The effect of in vitro stimulation of rat parotid gland with the neuropeptides substance P, calcitonin gene-related peptide and galanin has been studied by microfi lament fl uorescence staining and in semithin sections, and compared to control incubations and in vitro stimulation with b-adrenergic and muscarinic agonists. Clear-cut aspects of massive granule exocytosis and cytoplasm vacuolation, indicative of protein and fl uid secretion respectively, were obvious only after substance P stimulation, whereas treatment with galanin and calcitonin gene-related peptide produced little to no morphological changes. The results being in agreement with the outcome of other methodological approaches, these procedures appear reliable, may be effectively applied to the study of the functional regulation of secretory mechanisms, and may be particularly useful in human tissue analyses.
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