Mitotic and meiotic chromosomes of the crayfish Procambarus clarkii (Girard, 1852) were characterized by means of heterochromatin staining techniques (C-, DAPI- and CMA(3)-bandings), and FISH of major ribosomal genes and telomeric pentameric repeat (TTAGG)(n). Nucleolar organizer regions have been located on four chromosome pairs. GC-rich heterochromatin was mostly associated with NORs, and brightly fluorescent centromeric/pericentromeric AT-rich bands were present in most chromosomes. Telomeric pentameric repeats (TTAGG)(n), occurred in all the P. clarkii telomeres and in the interstitial region of the largest chromosome pair, not co-located to ribosomal genes; as possible explanation of the origin of this chromosome, a fusion event was hypothesized.

Karyotype, ribosomal genes, and telomeric sequences in the crayfish Procambarus clarkii (Decapoda: Cambaridae)

SALVADORI, SUSANNA;COLUCCIA, ELISABETTA;DEIDDA, FEDERICA;CAU, ANGELO;CANNAS, RITA;LOBINA, CINZIA;SABATINI, ANDREA;DEIANA, ANNA MARIA
2014-01-01

Abstract

Mitotic and meiotic chromosomes of the crayfish Procambarus clarkii (Girard, 1852) were characterized by means of heterochromatin staining techniques (C-, DAPI- and CMA(3)-bandings), and FISH of major ribosomal genes and telomeric pentameric repeat (TTAGG)(n). Nucleolar organizer regions have been located on four chromosome pairs. GC-rich heterochromatin was mostly associated with NORs, and brightly fluorescent centromeric/pericentromeric AT-rich bands were present in most chromosomes. Telomeric pentameric repeats (TTAGG)(n), occurred in all the P. clarkii telomeres and in the interstitial region of the largest chromosome pair, not co-located to ribosomal genes; as possible explanation of the origin of this chromosome, a fusion event was hypothesized.
2014
Cambaridae; Decapoda; Ribosomal genes; Telomeres
File in questo prodotto:
File Dimensione Formato  
JCB_2247_Salvadori.pdf

Solo gestori archivio

Tipologia: versione editoriale
Dimensione 8.59 MB
Formato Adobe PDF
8.59 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/98919
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 14
social impact