2026-09-24T04:43:30Zhttps://iris.unica.it/oai/requestoai:iris.unica.it:11584/2668092022-10-15T19:57:45Zcom_11584_207615com_11584_111066col_11584_265854
Studio di componenti del lattice di Euphorbia characias
MASCIA, CLAUDIA
Euphorbia characias
acetylcholinest
antiossidanti
antioxidants
cis-preniltrasferasi
gomma naturale
inibitori della acetilcolinesterosi
latex
lattice
Settore BIO/10 - Biochimica
The aim of the present study was to contribute to the knowledge of the
components of the latex, a milky fluid exudated from the Mediterranean
shrub Euphorbia characias.
The antioxidant and acetylcholinesterase inhibitory activities were explored
in E. characias. The latex was extracted using several conventional methods
and a new procedure involving the use of the trichloroacetic acid. The
results of this research indicate that E. characias latex exhibits antioxidant
activities, is rich in total polyphenolic and flavonoids content and exhibited
substantial inhibition of acetylcholinesterase activity.
A natural rubber was identified and characterized for the first time in the
latex of E. characias. Four different methods, i.e. acetone, acetic acid,
trichloroacetic acid and Triton® X-100, followed by successive treatments
with cyclohexane/ethanol, were employed to extract the natural rubber. The
higher yield (14%) was achieved after extraction with acetic acid. E.
characias rubber showed a molecular weight of 93000 with a Mw/Mn of 2.9.
1HNMR, 13C NMR and FTIR analysis revealed the characteristic of the cis-
1,4-polyisoprene typical of natural rubber.
The enzyme catalyzing the rubber molecule elongation is cisprenyltransferase
called “rubber transferase”. We cloned the cDNA
encoding for the E. characias rubber transferase by RT-PCR. The cDNA
contains an open reading frame of 810 bp encoding a protein of 270 amino
acids (GenBank JX564541). The cDNA nucleotide sequence and the
deduced amino acid sequence appear to be highly homologous to the
sequence of several plant cis-prenyltransferases.
The results provided novel insight into latex components and will ultimately
benefit the broader understanding of E. characias latex composition.
2015-05-26
info:eu-repo/semantics/doctoralThesis
http://hdl.handle.net/11584/266809
ita
numberofpages:103
info:eu-repo/semantics/openAccess
Università degli Studi di Cagliari
license:Non specificato