Immunohistochemical markers of stem/progenitor cells in the developing human cerebellum

The aim of this study was to identify the stem/progenitor cell markers, by immunohistochemistry, in order to highlight the cortical neurogenesis niches during the different gestational ages. To this end, the following stem/progenitor cell markers have been utilized: Sox2, Pax6, Pax2, WT1, Nestin, Vimentin and Calretinin. The expression of these markers have been compared with the expression of those markers of mature neurons and glial markers including Neurofilament (NF), neuron specific enolase (NSE), Synaptophysin (Syn), Glial fibrillary acid protein (GFAP) and S100β. Evaluating both stem/progenitor and mature cell markers lead to the identification of multiple stages of differentiation of neuronal and glial progenitors during gestation, in order to better understand the development of human cerebellar cortex.