Type-I diabetes leads to cardiomyopathy, which has been also ascribed to alterations in mitochondrial physiology. Despite numerous investigations on this topic it is still not clear what mechanisms develop mitochondrial impairment. We investigated on bioenergetic, morphological and proteomic mitochondrial features in a rat model of type-I diabetes. Female Wistar rats were injected with 60mg/kg streptozotocin and after 19-20 weeks of diabetic condition we checked left or right ventricles (LV or RV) mitochondrial subsarcolemmal (SSM) or interfibrillar (IFM) subpopulations, for changes in: activity of fatty acid import enzymes, oxidative phosphorylation (OXPHOS), cristal ultrastructure, mitofilin and Connexin 43 content. In our animal model of type-I diabetes there is a mild impairment of OXPHOS in different complexes for IFM and SSM, but this is not due to alteration of ion homeostasis (same Cx43). Mitofilin decrease is not enough to result in a manifest cristae morphology alteration, but might indicate a start of cristae reorganization, which is not yet significant after 5 months of diabetes. Surprisingly, both SSM (especially RV ones) seemed to be more sensitive to higher lipid input since their OXPHOS is defective. Lastly, diabetic RV SSM impairment might lead to cardiomyopathy of RV, recently reported in Type-II diabetes.

Diabetes causes right ventricle mitochondria impairment

R. Vargiu
Investigation
;
A. Casti
Membro del Collaboration Group
;
F. Broccia
Membro del Collaboration Group
;
F. Loy
Membro del Collaboration Group
;
M. Isola
Membro del Collaboration Group
;
R. Isola
Writing – Original Draft Preparation
2019-01-01

Abstract

Type-I diabetes leads to cardiomyopathy, which has been also ascribed to alterations in mitochondrial physiology. Despite numerous investigations on this topic it is still not clear what mechanisms develop mitochondrial impairment. We investigated on bioenergetic, morphological and proteomic mitochondrial features in a rat model of type-I diabetes. Female Wistar rats were injected with 60mg/kg streptozotocin and after 19-20 weeks of diabetic condition we checked left or right ventricles (LV or RV) mitochondrial subsarcolemmal (SSM) or interfibrillar (IFM) subpopulations, for changes in: activity of fatty acid import enzymes, oxidative phosphorylation (OXPHOS), cristal ultrastructure, mitofilin and Connexin 43 content. In our animal model of type-I diabetes there is a mild impairment of OXPHOS in different complexes for IFM and SSM, but this is not due to alteration of ion homeostasis (same Cx43). Mitofilin decrease is not enough to result in a manifest cristae morphology alteration, but might indicate a start of cristae reorganization, which is not yet significant after 5 months of diabetes. Surprisingly, both SSM (especially RV ones) seemed to be more sensitive to higher lipid input since their OXPHOS is defective. Lastly, diabetic RV SSM impairment might lead to cardiomyopathy of RV, recently reported in Type-II diabetes.
Mitochondria. OXPHOS, mitochondrial criste, type-I diabetes
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/273879
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