System for the simultaneous detection and identification in a single miniaturized solid support (chip) of pathogenic agents that are detectable in foodstuffs, water or in biological samples such as, for example, swabs, biopsies, blood and faeces. The chip is comprised in a kit based on a method that provides for an extraction of the nucleic acid (DNA or RNA) in the sample, an amplification of specific genic sequences, whether or not preceded by Reverse Transcription (RT) if the target nucleic acid is an RNA, and a hybridization of the amplification product by specific probes comprised in the chip.
System for searching for and identifying pathogenic agents
DE MONTIS, ANTONELLA;
2005-01-01
Abstract
System for the simultaneous detection and identification in a single miniaturized solid support (chip) of pathogenic agents that are detectable in foodstuffs, water or in biological samples such as, for example, swabs, biopsies, blood and faeces. The chip is comprised in a kit based on a method that provides for an extraction of the nucleic acid (DNA or RNA) in the sample, an amplification of specific genic sequences, whether or not preceded by Reverse Transcription (RT) if the target nucleic acid is an RNA, and a hybridization of the amplification product by specific probes comprised in the chip.
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