Objective: In the present review article we have summarized the use of the metabolomics approach to study the metabolic modifications occurring in several bio-fluids due to viral infections. The aim is to highlight the ability of metabolomics to find early fingerprints, which are related to the infections. Methods: The 1H-NMR, UHPLC/MS/MS2, UPLC/ESI-SYNAPT-HDMS, UPLC-Q-TOF-HDMS analyses were used for the determination of several metabolites representative of the viral infections. The data were analyzed by Principal Component Analysis (PCA), Partial Least Square Discriminant Analysis (PLS-DA) and by regression techniques. Results: The major changes were related to nucleotide, carbohydrates, lipids and amino acid metabolisms. Conclusions: The metabolomics approach could be considered a viable option for characterization of the viral infection and for detecting on going differences in the bio-fluids composition.

Metabolomics technology and their application to the study of the viral infection

NOTO, ANTONIO;DESSI', ANGELICA;FANOS, VASSILIOS
2014

Abstract

Objective: In the present review article we have summarized the use of the metabolomics approach to study the metabolic modifications occurring in several bio-fluids due to viral infections. The aim is to highlight the ability of metabolomics to find early fingerprints, which are related to the infections. Methods: The 1H-NMR, UHPLC/MS/MS2, UPLC/ESI-SYNAPT-HDMS, UPLC-Q-TOF-HDMS analyses were used for the determination of several metabolites representative of the viral infections. The data were analyzed by Principal Component Analysis (PCA), Partial Least Square Discriminant Analysis (PLS-DA) and by regression techniques. Results: The major changes were related to nucleotide, carbohydrates, lipids and amino acid metabolisms. Conclusions: The metabolomics approach could be considered a viable option for characterization of the viral infection and for detecting on going differences in the bio-fluids composition.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/101170
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