The aim of this work was to ascertain the capability of a commercial mixture of decylpolyglucoside (Oramix NS10) to form vesicle structures in water, either alone or in association with cholesterol. To this purpose we compared results obtained from a theoretical model with those acquired from the experimental characterization of different Oramix NS10/cholesterol mixtures. The germen vesicular formation theory was used to predict the formation process of vesicular structures. To this purpose, the energy balance involved in the vesicle formation was calculated using critical concentration for vesicle formation (ccf), surface tension and molecular area of decylpolyglucoside. The ccf was measured by turbidity measurements, and surface tension was determined by a tensiometer study. In order to confirm the validity of the theoretical model, we prepared and characterized vesicles made with decylpolyglucoside and cholesterol in several molar ratios and using different preparation methods. Vesicles were characterized using optical and light polarized microscopy, transmission electron microscopy and dynamic light scattering. The capability of vesicles to encapsulate a hydrophilic probe was also studied. Results obtained showed that the decylpolyglucoside surfactant is able to form vesicles only; in the presence of an appropriate cholesterol content (i.e. Oramix NS10/cholesterol molar ratio >= 1/0.4) and that the mathematical model proposed is a useful and reproducible toot capable of predicting the type of aggregation structures given by the alkylpolyglucoside in water.
Theoretical and experimental evaluation of decypolyglucoside vesicles as potential drug delivery systems
MANCONI, MARIA;SINICO, CHIARA;FADDA, ANNA MARIA
2006-01-01
Abstract
The aim of this work was to ascertain the capability of a commercial mixture of decylpolyglucoside (Oramix NS10) to form vesicle structures in water, either alone or in association with cholesterol. To this purpose we compared results obtained from a theoretical model with those acquired from the experimental characterization of different Oramix NS10/cholesterol mixtures. The germen vesicular formation theory was used to predict the formation process of vesicular structures. To this purpose, the energy balance involved in the vesicle formation was calculated using critical concentration for vesicle formation (ccf), surface tension and molecular area of decylpolyglucoside. The ccf was measured by turbidity measurements, and surface tension was determined by a tensiometer study. In order to confirm the validity of the theoretical model, we prepared and characterized vesicles made with decylpolyglucoside and cholesterol in several molar ratios and using different preparation methods. Vesicles were characterized using optical and light polarized microscopy, transmission electron microscopy and dynamic light scattering. The capability of vesicles to encapsulate a hydrophilic probe was also studied. Results obtained showed that the decylpolyglucoside surfactant is able to form vesicles only; in the presence of an appropriate cholesterol content (i.e. Oramix NS10/cholesterol molar ratio >= 1/0.4) and that the mathematical model proposed is a useful and reproducible toot capable of predicting the type of aggregation structures given by the alkylpolyglucoside in water.
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