Study of nucleolar organizer region in Palinurus elephas (Crustacea, Decapoda)

Using fluorescence in situ hybridization (FISH), chromomycin (CMA 3) staining and silver staining, we studied the nucleolar organizer regions in the spiny lobster Palinurus elephas in order to extend our knowledge on the karyology of this commercially important species. Multiple NORs have been detected by FISH, and CMA3 showed a good correspondence between the localization of GC-rich heterochromatin and the ribosomal genes mapped by FISH. In contrast, the number of Ag-positive regions was higher than the number of FISH and CMA3 signals, which may be explained by silver staining of the kinetochores. A variability in the number of FISH and CMA3 signals has been detected in metaphases I and II which is probably due to the occurrence of rDNA cistrons on B chromosomes