Our study examines the functional and structural effects of amino acid substitution in the distal side of b-chains of human Hb Duarte (a b62Ala/Pro). We have compared the functional properties of the purified Hb Duarte with those of HbA, and 22 through proton NMR and molecular dynamics simulations we have investigated their tertiary and quaternary structures. The variant exhibits an increased oxygen affinity with a normal Hill coefficient and Bohr effect. The abnormal function of Hb Duarte is attributed to the presence of a proline residue at the b62 position, since the functional properties of another Hb variant in the same position, Hb J-Europa (b62Ala/Asp), have been described as normal. Thereafter 1H-NMR studies have shown that the b62 Ala/Pro substitution causes structural modifications of the tertiary structure of the b globins, leaving the quaternary structure unaltered. These results have been confirmed by extensive all-atom molecular dynamics simulations. All these findings lead to the conclusion that the b62 Ala/Pro substitution produces a destabilization of the E-helix extending downward to the CD corner. Particularly, a cavity near the distal histidine of the b-chains, connecting the heme pocket to the solvent, is affected, altering the functional properties of the protein molecule.

Structure-function relationship in a variant haemoglobin: a combined computational-experimental approach

CECCARELLI, MATTEO;RUGGERONE, PAOLO;FAIS, ANTONELLA;ERA, BENEDETTA;CASU, MARIANO
2006-01-01

Abstract

Our study examines the functional and structural effects of amino acid substitution in the distal side of b-chains of human Hb Duarte (a b62Ala/Pro). We have compared the functional properties of the purified Hb Duarte with those of HbA, and 22 through proton NMR and molecular dynamics simulations we have investigated their tertiary and quaternary structures. The variant exhibits an increased oxygen affinity with a normal Hill coefficient and Bohr effect. The abnormal function of Hb Duarte is attributed to the presence of a proline residue at the b62 position, since the functional properties of another Hb variant in the same position, Hb J-Europa (b62Ala/Asp), have been described as normal. Thereafter 1H-NMR studies have shown that the b62 Ala/Pro substitution causes structural modifications of the tertiary structure of the b globins, leaving the quaternary structure unaltered. These results have been confirmed by extensive all-atom molecular dynamics simulations. All these findings lead to the conclusion that the b62 Ala/Pro substitution produces a destabilization of the E-helix extending downward to the CD corner. Particularly, a cavity near the distal histidine of the b-chains, connecting the heme pocket to the solvent, is affected, altering the functional properties of the protein molecule.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/108885
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