A simple HPLC method was developed to distinguish between ‘poisonous’ and ‘nonpoisonous’ chemotypes of Ferula communis. The method was performed on a C8 reverse phase analytical column usinga binary eluent (aqueous TFA 0.01%–TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives by HPLC-diode array detection (HPLC-DAD) and HPLC-MS is described. A coumarin, not yet described, was detected.
HPLC-DAD-MS identification of bioactive secondary metabolites from Ferula communis roots
BALLERO, MAURO;MAXIA, ANDREA;
2004-01-01
Abstract
A simple HPLC method was developed to distinguish between ‘poisonous’ and ‘nonpoisonous’ chemotypes of Ferula communis. The method was performed on a C8 reverse phase analytical column usinga binary eluent (aqueous TFA 0.01%–TFA 0.01% in acetonitrile) under gradient condition. The two chemotypes showed different fingerprints. The identification of five coumarins and eleven daucane derivatives by HPLC-diode array detection (HPLC-DAD) and HPLC-MS is described. A coumarin, not yet described, was detected.
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