Porous silicon-based potentiometric biosensor for triglycerides

In this paper we report on the fabrication and characterization of a potentiometric biosensor for the detec- tion of triglycerides. This is constituted by a lipase immobilized on a mesoporous Si matrix. Prototypes, realized on 1 × 1 cm n+ -type silicon wafers, show a very high enzymatic activity. Moreover the properties of these biosensors have been shown to be stable in a several months time interval, clearly showing their advantages with respect to traditional triglycerides detection systems. The Michaelis Menten curve is ob- tained to demonstrate the absence of diffusion problems. Potentiometric measurements are also shown.