UNICA IRIS Institutional Research Information System
IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Cagliari dal mese di luglio 2015.
EnglishA method for separation and quantification of S-nitrosoglutathione in red cell extracts by capillary electrophoresis is reported. The method is based on the direct analysis of the metaphosphoric acid erythrocyte extract containing diethylenetriaminepentaacetic acid. Optimization of the method is briefly discussed. Best results in the shortest time were obtained at 25°C, using a coated capillary, 7 kV applied voltage and phosphate sodium 40 mmol/L (pH 2.2) as running buffer. Reproducibility, detection limits, and recoveries of S-nitrosoglutathione analyses were checked. The results evidenced that S- nitrosoglutathione is formed in erythrocytes treated with S-nitrosocysteine, a transnitrosating agent. Under our experimental conditions, the contemporaneous detection and quantification of reduced and oxidized glutathione present in cell extract could also be performed.
Determination of S-nitrosoglutathione in erythrocytes by capillary zone electrophoresis / MESSANA I; ROSSETTI D.V.; MISITI F.; VINCENZONI F.; TELLONE E.; GIARDINA B.; CASTAGNOLA M.. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 21:8(2000), pp. 1606-1610.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
| Titolo: | Determination of S-nitrosoglutathione in erythrocytes by capillary zone electrophoresis |
| Autori: | |
| Data di pubblicazione: | 2000 |
| Rivista: | |
| Citazione: | Determination of S-nitrosoglutathione in erythrocytes by capillary zone electrophoresis / MESSANA I; ROSSETTI D.V.; MISITI F.; VINCENZONI F.; TELLONE E.; GIARDINA B.; CASTAGNOLA M.. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 21:8(2000), pp. 1606-1610. |
| Abstract: | A method for separation and quantification of S-nitrosoglutathione in red cell extracts by capillary electrophoresis is reported. The method is based on the direct analysis of the metaphosphoric acid erythrocyte extract containing diethylenetriaminepentaacetic acid. Optimization of the method is briefly discussed. Best results in the shortest time were obtained at 25°C, using a coated capillary, 7 kV applied voltage and phosphate sodium 40 mmol/L (pH 2.2) as running buffer. Reproducibility, detection limits, and recoveries of S-nitrosoglutathione analyses were checked. The results evidenced that S- nitrosoglutathione is formed in erythrocytes treated with S-nitrosocysteine, a transnitrosating agent. Under our experimental conditions, the contemporaneous detection and quantification of reduced and oxidized glutathione present in cell extract could also be performed. |
| Handle: | http://hdl.handle.net/11584/1189 |
| Tipologia: | 1.1 Articolo in rivista |
File in questo prodotto:
Copyright © 2020