The use of a polyclonal antiserum specific to C-terminal tetrapeptide amide of (D-Ala(2))deltorphin-I, a naturally occurring amphibian skin opioid peptide, has already demonstrated the presence of immunoreactive neurons in rat midbrain. Double immunostaining identified these neurons as a sub-population of the mesencephalic dopaminergic neurons that were also tyrosine hydroxylase-immunopositive and calbindin-D28kD- negative, namely, the neurons predominantly affected in Parkinson disease. We followed the fate of these neurons after a monolateral injection of 6-hydroxy-dopamine into rat brain. Almost all the immunopositive neurons and their nigrostriatal, mesolimbic and mesocortical projections on the side ipsilateral to the lesion disappeared. Only a few scattered immunopositive neurons within the substantia nigra, pars compacta, and those of supramammillary nucleus remained unaffected. The consistent overlap of dopamine and this new molecule provides a further key to identifying the mammalian counterpart of these amphibian skin opioid peptides.

Fate of (D-Ala2)-deltorphin-I-like immunoreactive neurons in 6-hydroxydopamine lesioned rat brain

DI CHIARA, GAETANO;
2004

Abstract

The use of a polyclonal antiserum specific to C-terminal tetrapeptide amide of (D-Ala(2))deltorphin-I, a naturally occurring amphibian skin opioid peptide, has already demonstrated the presence of immunoreactive neurons in rat midbrain. Double immunostaining identified these neurons as a sub-population of the mesencephalic dopaminergic neurons that were also tyrosine hydroxylase-immunopositive and calbindin-D28kD- negative, namely, the neurons predominantly affected in Parkinson disease. We followed the fate of these neurons after a monolateral injection of 6-hydroxy-dopamine into rat brain. Almost all the immunopositive neurons and their nigrostriatal, mesolimbic and mesocortical projections on the side ipsilateral to the lesion disappeared. Only a few scattered immunopositive neurons within the substantia nigra, pars compacta, and those of supramammillary nucleus remained unaffected. The consistent overlap of dopamine and this new molecule provides a further key to identifying the mammalian counterpart of these amphibian skin opioid peptides.
amphibian skin peptides, opioid peptides, delta-opioid receptors, dopaminergic system, tyrosine hydroxylase, calbindin-D28kD, Parkinson disease ; I-LIKE IMMUNOREACTIVITY, DELTA-OPIOID RECEPTORS, IMMUNOHISTOCHEMICAL DEMONSTRATION, EPITHELIAL-CELLS, DELTORPHIN-I, DERMORPHIN, PEPTIDE, SKIN, LOCALIZATION, EXPRESSION
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/12412
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