Pericytes stabilize vessel wall, participate in the regulation of blood flow microcirculation and influence endothelial proliferation, survival, migration and maturation. Pericytes are commonly identified by molecular markers, such as desmin. The aim of this study was to analyze the pattern of distribution of desmin in the chick embryo chorioallantoic membrane (CAM) vasculature in normal condition and in response to an angiogenic cytokine, such as fibroblast growth factor-2 (FGF-2) by using conventional immunohistochemistry and confocal laser scanning microscopy. Results indicate that, in normal condition, the desmin-positive pericytes are located around the endothelial cells of the capillary plexus and of larger vessels in the intermediate mesenchyme. In response to FGF-2 numerous desmin-positive and a smooth muscle actin-negative cells are located in the mesenchyme and numerous blood vessels positive to factor VIII and desmin are recognizable near to the gelatin sponge soaked with FGF-2. These data suggest that pericytes in normal condition intervene to stabilize the wall of newly formed blood vessel, while in response to FGF-2, they detach from the wall of parental vessels and migrate, as extramural fibroblast-like desmin-positive cells, in the surrounding mesenchyme, where they modulate the angiogenic response. (C) 2004 Elsevier Inc. All rights reserved.

Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2

ENNAS, MARIA GRAZIA;
2004-01-01

Abstract

Pericytes stabilize vessel wall, participate in the regulation of blood flow microcirculation and influence endothelial proliferation, survival, migration and maturation. Pericytes are commonly identified by molecular markers, such as desmin. The aim of this study was to analyze the pattern of distribution of desmin in the chick embryo chorioallantoic membrane (CAM) vasculature in normal condition and in response to an angiogenic cytokine, such as fibroblast growth factor-2 (FGF-2) by using conventional immunohistochemistry and confocal laser scanning microscopy. Results indicate that, in normal condition, the desmin-positive pericytes are located around the endothelial cells of the capillary plexus and of larger vessels in the intermediate mesenchyme. In response to FGF-2 numerous desmin-positive and a smooth muscle actin-negative cells are located in the mesenchyme and numerous blood vessels positive to factor VIII and desmin are recognizable near to the gelatin sponge soaked with FGF-2. These data suggest that pericytes in normal condition intervene to stabilize the wall of newly formed blood vessel, while in response to FGF-2, they detach from the wall of parental vessels and migrate, as extramural fibroblast-like desmin-positive cells, in the surrounding mesenchyme, where they modulate the angiogenic response. (C) 2004 Elsevier Inc. All rights reserved.
2004
Angiogenesis; FGF2; desmin
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/12771
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 18
social impact