To verify the serological response mounted against antigenic peptides from HERV-Wenv protein, we analyzed 80 multiple sclerosis (MS) serum samples, 27 of which were re-analyzed after a 6-month follow-up IFN-β therapy, and 73 healthy controls. Indirect ELISAs were carried out to detect antibodies specific for all the synthetic peptides derived from HERV-Wenv. Two antigenic peptides, HERV-Wenv<inf>93-108</inf> (31.25%, p<0.0001) and HERV-Wenv<inf>248-262</inf> (15%, p=0.02), were highly recognized by MS patients' antibodies when compared to healthy subjects. Moreover, antibody titer against these two peptides slightly decreased after six months of IFN-β-based therapy.

Epitopes of HERV-Wenv induce antigen-specific humoral immunity in multiple sclerosis patients

COCCO, ELEONORA;
2015

Abstract

To verify the serological response mounted against antigenic peptides from HERV-Wenv protein, we analyzed 80 multiple sclerosis (MS) serum samples, 27 of which were re-analyzed after a 6-month follow-up IFN-β therapy, and 73 healthy controls. Indirect ELISAs were carried out to detect antibodies specific for all the synthetic peptides derived from HERV-Wenv. Two antigenic peptides, HERV-Wenv93-108 (31.25%, p<0.0001) and HERV-Wenv248-262 (15%, p=0.02), were highly recognized by MS patients' antibodies when compared to healthy subjects. Moreover, antibody titer against these two peptides slightly decreased after six months of IFN-β-based therapy.
HERV-Wenv; Humoral response; IFN-β therapy; Adult; Antibodies, viral; Case-control studies; Endogenous retroviruses; Enzyme-linked immunosorbent assay; Epitopes; Female; Humans; Immunity, humoral; Male; Middle aged; Multiple sclerosis; Peptides; Immunology; Neurology (clinical); Immunology and allergy; Neurology; Medicine (all)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11584/179604
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