The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H2O2 scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H2O2 and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2¢-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.

Hofmeister Effects in Biology: Effect of Choline addition on the salt-induced Superactivity of HRP and its implicatiopn for salt resistance of Plant

MONDUZZI, MAURA;
2005-01-01

Abstract

The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H2O2 scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H2O2 and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2¢-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.
2005
Hofmeister; Enzyme superactivity; Ion specific effects
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/18988
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