In the present paper a method for the extraction of Agaricus bisporus tyrosinase, based on the use of a diafiltration procedure in the presence of ascorbate, is proposed. During the extraction of tyrosinase the oxidation of mushroom phenolics was avoided, keeping phenolic compounds in their reduced form throughout the extraction step. Their complete removal by means of a diafiltration apparatus was achieved. The method here described represents a useful way to obtain an enzymic solution of tyrosinase, devoid of phenolics and melanic compounds.
Diafiltration in the presence of ascorbate in the purification of mushroom tyrosinase
RESCIGNO, ANTONIO;SANJUST, ENRICO;RINALDI, ANDREA;CURRELI, NICOLETTA;
1997-01-01
Abstract
In the present paper a method for the extraction of Agaricus bisporus tyrosinase, based on the use of a diafiltration procedure in the presence of ascorbate, is proposed. During the extraction of tyrosinase the oxidation of mushroom phenolics was avoided, keeping phenolic compounds in their reduced form throughout the extraction step. Their complete removal by means of a diafiltration apparatus was achieved. The method here described represents a useful way to obtain an enzymic solution of tyrosinase, devoid of phenolics and melanic compounds.
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