In the present paper a method for the extraction of Agaricus bisporus tyrosinase, based on the use of a diafiltration procedure in the presence of ascorbate, is proposed. During the extraction of tyrosinase the oxidation of mushroom phenolics was avoided, keeping phenolic compounds in their reduced form throughout the extraction step. Their complete removal by means of a diafiltration apparatus was achieved. The method here described represents a useful way to obtain an enzymic solution of tyrosinase, devoid of phenolics and melanic compounds.

Diafiltration in the presence of ascorbate in the purification of mushroom tyrosinase

RESCIGNO, ANTONIO;SANJUST, ENRICO;RINALDI, ANDREA;CURRELI, NICOLETTA;
1997-01-01

Abstract

In the present paper a method for the extraction of Agaricus bisporus tyrosinase, based on the use of a diafiltration procedure in the presence of ascorbate, is proposed. During the extraction of tyrosinase the oxidation of mushroom phenolics was avoided, keeping phenolic compounds in their reduced form throughout the extraction step. Their complete removal by means of a diafiltration apparatus was achieved. The method here described represents a useful way to obtain an enzymic solution of tyrosinase, devoid of phenolics and melanic compounds.
1997
Tyrosinase; Protein purification; Agaricus bisporus
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/2525
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact