Tyrosine-hydroxylase, dopamine β-hydroxylase and choline acetyltransferase-like immunoreactive fibres in the human major sublingual gland

Objective: To study the innervation of the major sublingual gland by means of immunohistochemistry. Design: Bioptic and autoptic specimens of the major sublingual gland of humans were examined for the presence of immunoreactivity to tyrosine hydroxylase and dopamine-β-hydroxylase, on one hand, and choline acetyltransferase, on the other, to indicate adrenergic and cholinergic nerves, respectively. Results: Acini and ducts were supplied by both divisions of the autonomic nervous system. Conclusions: Mucous and seromucous cells of the human major sublingual glands may respond with secretion not only to parasympathetic activity but also to sympathetic activity. The major sublingual gland is therefore a potential contributor to the mucin secretion recently reported in the literature in response to high sympathetic activity during physical exercise.