We here exploit pH-responsive nanogels as carriers to deliver functional anti-GFP siRNA and superparamagnetic IONPs to HeLa-GFP cells. The siRNA release via pH-mediated endosomal escape is shown. The IONPs act first as magnetofection agents to boost cellular uptake and then as probes to track the release mechanism by electron microscopy.
Magnetic pH-responsive nanogels as multifunctional delivery tools for small interfering RNA (siRNA) molecules and iron oxide nanoparticles (IONPs)
FALQUI, ANDREA;
2012-01-01
Abstract
We here exploit pH-responsive nanogels as carriers to deliver functional anti-GFP siRNA and superparamagnetic IONPs to HeLa-GFP cells. The siRNA release via pH-mediated endosomal escape is shown. The IONPs act first as magnetofection agents to boost cellular uptake and then as probes to track the release mechanism by electron microscopy.
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