The DNA sequence d(GGGGTTTTGGGG) consists of 1.5 units of the repeat in telomeres of Oxytricha nova. It has been shown by NMR and x-ray crystallographic analysis that it is capable to form a dimeric quadruplex structure and that a variety of cations, namety K+, Na+, and NH4+, are able to interact with this complex with different affinity, leading to complexes characterized by different local conformations. Thus, in order to improve the knowledge of this kind of molecule, and in particular to Providejurther insight into the role of monovalent cations in the G-quadruplex folding and conformation, we have investigated by H-1-NMR the effect of the addition of Rb+ and Cs+ to the quadruplex formed by the oligonucleotide d(GGGGTTTTGGGG).
Effect of rubidium and cesium ions on the dimeric quaduplex formed by the Oxytricha nova telomeric repeat oligonucleotide d(GGGGTTTTGGGG)
CESARE MARINCOLA, FLAMINIA;
2007-01-01
Abstract
The DNA sequence d(GGGGTTTTGGGG) consists of 1.5 units of the repeat in telomeres of Oxytricha nova. It has been shown by NMR and x-ray crystallographic analysis that it is capable to form a dimeric quadruplex structure and that a variety of cations, namety K+, Na+, and NH4+, are able to interact with this complex with different affinity, leading to complexes characterized by different local conformations. Thus, in order to improve the knowledge of this kind of molecule, and in particular to Providejurther insight into the role of monovalent cations in the G-quadruplex folding and conformation, we have investigated by H-1-NMR the effect of the addition of Rb+ and Cs+ to the quadruplex formed by the oligonucleotide d(GGGGTTTTGGGG).
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