The thiol status of several human normal cells, including human bronchial epithelial cells and fibroblasts, oral fibroblasts and skin fibroblasts, has been investigated. Reduced glutathione (GSH) has been confirmed as the most abundant thiol. The other low molecular weight thiols were in the following order: cysteine> cystine> oxidised glutathione. Differences were detected also in protein sulfhydryl groups. Bronchial epithelial cells had the highest level of GSH. The lowest content was in oral fibroblast. The data show differences in the content of thiols groups between human normal cells in culture. Due to the role of thiols in pathophysiological processes these differences should be considered in order to interpret correctly results obtained in in vitro studies.
Differences in thiol status of cultured normal human cells
ATZORI, LUIGI
1996-01-01
Abstract
The thiol status of several human normal cells, including human bronchial epithelial cells and fibroblasts, oral fibroblasts and skin fibroblasts, has been investigated. Reduced glutathione (GSH) has been confirmed as the most abundant thiol. The other low molecular weight thiols were in the following order: cysteine> cystine> oxidised glutathione. Differences were detected also in protein sulfhydryl groups. Bronchial epithelial cells had the highest level of GSH. The lowest content was in oral fibroblast. The data show differences in the content of thiols groups between human normal cells in culture. Due to the role of thiols in pathophysiological processes these differences should be considered in order to interpret correctly results obtained in in vitro studies.
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