Lipids and coumarin extraction from cumaru seeds (Dipteryx odorata) using sequential supercritical CO2+solvent and pressurized ethanol

Lipids and coumarin were extracted from cumaru seeds using Soxhlet extraction (SE) with ethanol (EtOH) and ethyl acetate (EtOAc), scCO2, scCO2 +EtOH and scCO2 +EtOAc, either in a single or in sequential stages, and with PLE with EtOH. ScCO2 +EtOAc provided yields from 28% to 31%, independently of the extraction conditions employed, and scCO2 +EtOH provide a top yield of 38%, at 25 MPa and 80 ºC. Extraction yields were enhanced by three sequential extractions, resulting in yields of 44.0% and 56.5% for scCO2 +EtOAc and scCO2 +EtOH, respectively, where these values were superior to those obtained in SE. This work also reported the kinetics of coumarin extraction using scCO2 +cosolvents, where in the case of scCO2 +EtOH the coumarin content was 30 mg g−1 of extract. Finally, the coumarin crystals seemed to be affected by the cosolvent nature, as indicated by the higher antioxidant activity of coumarin-rich extracts obtained by scCO2 +EtOAc, rather than those obtained by scCO2 +EtOH.