UNICA IRIS Institutional Research Information System
IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Cagliari dal mese di luglio 2015.
EnglishFluorescent probes are currently used to evaluate the mitochondrial transmembrane potential in situ, However, in parallel experiments using the probes JC-1 and TMRM in different cell types (human astrocytes, HEp-2, Vero, KB, and HeLa tells), we found that the distribution of JC-1 and TMRM is highly variable not only in different tell types but also in different cells of the same cell type, a condition that has never been documented until our work, This phenomenon depends on a hidden, widespread multidrug resistance (MDR) phenotype that can be recognized only by comparative assays with MDR inhibitors (progesterone, verapamil, and cyclosporin A) and represents a serious risk of error in the evaluation of the mitochondrial potential.
Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators / Diaz G; Diana A; Falchi A M; Gremo F; Pani A; Batetta B; Dessì S; Isola R. - 51:2(2001), pp. 121-126.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
| Titolo: | Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators | |
| Autori: | ||
| Data di pubblicazione: | 2001 | |
| Rivista: | ||
| Citazione: | Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators / Diaz G; Diana A; Falchi A M; Gremo F; Pani A; Batetta B; Dessì S; Isola R. - 51:2(2001), pp. 121-126. | |
| Abstract: | Fluorescent probes are currently used to evaluate the mitochondrial transmembrane potential in situ, However, in parallel experiments using the probes JC-1 and TMRM in different cell types (human astrocytes, HEp-2, Vero, KB, and HeLa tells), we found that the distribution of JC-1 and TMRM is highly variable not only in different tell types but also in different cells of the same cell type, a condition that has never been documented until our work, This phenomenon depends on a hidden, widespread multidrug resistance (MDR) phenotype that can be recognized only by comparative assays with MDR inhibitors (progesterone, verapamil, and cyclosporin A) and represents a serious risk of error in the evaluation of the mitochondrial potential. | |
| Handle: | http://hdl.handle.net/11584/5374 | |
| Tipologia: | 1.1 Articolo in rivista |
File in questo prodotto:
Copyright © 2022