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EnglishFluorescent probes are currently used to evaluate the mitochondrial transmembrane potential in situ, However, in parallel experiments using the probes JC-1 and TMRM in different cell types (human astrocytes, HEp-2, Vero, KB, and HeLa tells), we found that the distribution of JC-1 and TMRM is highly variable not only in different tell types but also in different cells of the same cell type, a condition that has never been documented until our work, This phenomenon depends on a hidden, widespread multidrug resistance (MDR) phenotype that can be recognized only by comparative assays with MDR inhibitors (progesterone, verapamil, and cyclosporin A) and represents a serious risk of error in the evaluation of the mitochondrial potential.
Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators / Diaz G; Diana A; Falchi A M; Gremo F; Pani A; Batetta B; Dessì S; Isola R. - 51:2(2001), pp. 121-126.
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| Titolo: | Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators | |
| Autori: | ||
| Data di pubblicazione: | 2001 | |
| Rivista: | ||
| Citazione: | Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators / Diaz G; Diana A; Falchi A M; Gremo F; Pani A; Batetta B; Dessì S; Isola R. - 51:2(2001), pp. 121-126. | |
| Abstract: | Fluorescent probes are currently used to evaluate the mitochondrial transmembrane potential in situ, However, in parallel experiments using the probes JC-1 and TMRM in different cell types (human astrocytes, HEp-2, Vero, KB, and HeLa tells), we found that the distribution of JC-1 and TMRM is highly variable not only in different tell types but also in different cells of the same cell type, a condition that has never been documented until our work, This phenomenon depends on a hidden, widespread multidrug resistance (MDR) phenotype that can be recognized only by comparative assays with MDR inhibitors (progesterone, verapamil, and cyclosporin A) and represents a serious risk of error in the evaluation of the mitochondrial potential. | |
| Handle: | http://hdl.handle.net/11584/5374 | |
| Tipologia: | 1.1 Articolo in rivista |
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