Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators

Fluorescent probes are currently used to evaluate the mitochondrial transmembrane potential in situ, However, in parallel experiments using the probes JC-1 and TMRM in different cell types (human astrocytes, HEp-2, Vero, KB, and HeLa tells), we found that the distribution of JC-1 and TMRM is highly variable not only in different tell types but also in different cells of the same cell type, a condition that has never been documented until our work, This phenomenon depends on a hidden, widespread multidrug resistance (MDR) phenotype that can be recognized only by comparative assays with MDR inhibitors (progesterone, verapamil, and cyclosporin A) and represents a serious risk of error in the evaluation of the mitochondrial potential.

Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators / Diaz G; Diana A; Falchi A M; Gremo F; Pani A; Batetta B; Dessì S; Isola R. - 51:2(2001), pp. 121-126.

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Titolo: Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators
Autori: 
DIAZ, GIACOMO
DIANA, ANDREA
FALCHI, ANGELA MARIA
PANI, ALESSANDRA
ISOLA, RAFFAELLA
mostra contributor esterni 
Data di pubblicazione: 2001
Rivista: 
IUBMB LIFE  
Citazione: Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators / Diaz G; Diana A; Falchi A M; Gremo F; Pani A; Batetta B; Dessì S; Isola R. - 51:2(2001), pp. 121-126.
Abstract: Fluorescent probes are currently used to evaluate the mitochondrial transmembrane potential in situ, However, in parallel experiments using the probes JC-1 and TMRM in different cell types (human astrocytes, HEp-2, Vero, KB, and HeLa tells), we found that the distribution of JC-1 and TMRM is highly variable not only in different tell types but also in different cells of the same cell type, a condition that has never been documented until our work, This phenomenon depends on a hidden, widespread multidrug resistance (MDR) phenotype that can be recognized only by comparative assays with MDR inhibitors (progesterone, verapamil, and cyclosporin A) and represents a serious risk of error in the evaluation of the mitochondrial potential.
Handle: http://hdl.handle.net/11584/5374
Tipologia:1.1 Articolo in rivista

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