Mesenchymal stem cells (MSCs) have the ability to differentiate into osteocytes, chondrocytes, and adipocytes and possess immunomodulatory properties. Amniotic membrane from human term placenta is a potential source of multipotent MSCs that could be useful for regenerative medicine. This unit describes a detailed and simple protocol for the isolation of amniotic mesenchymal cells. We also introduce a simple density separation technique for the purification of this cell type from possible contamination with amniotic epithelial cells.

Isolation of amniotic mesenchymal stem cells

MARONGIU, FABIO;
2010

Abstract

Mesenchymal stem cells (MSCs) have the ability to differentiate into osteocytes, chondrocytes, and adipocytes and possess immunomodulatory properties. Amniotic membrane from human term placenta is a potential source of multipotent MSCs that could be useful for regenerative medicine. This unit describes a detailed and simple protocol for the isolation of amniotic mesenchymal cells. We also introduce a simple density separation technique for the purification of this cell type from possible contamination with amniotic epithelial cells.
File in questo prodotto:
File Dimensione Formato  
2010 Marongiu (Isolation of Amniotic Mesenchymal Stem Cells).pdf

Solo gestori archivio

Tipologia: versione editoriale
Dimensione 444.8 kB
Formato Adobe PDF
444.8 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11584/73905
Citazioni
  • ???jsp.display-item.citation.pmc??? 24
  • Scopus 67
  • ???jsp.display-item.citation.isi??? 2
social impact