Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds(1). Caged compounds are molecules made physiologically inactive by a chemical cage that can be broken by a flash of ultraviolet light. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged compounds for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.
Flash photolysis of caged compounds in the cilia of olfactory sensory neurons
SAGHEDDU, CLAUDIA;
2011-01-01
Abstract
Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds(1). Caged compounds are molecules made physiologically inactive by a chemical cage that can be broken by a flash of ultraviolet light. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged compounds for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.
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