SUMMARY We have identified and quantified a Mycobacterium avium subsp. silvaticum (MAS) 1612 insertion sequence in ovine clinical samples, derived from two different sheep farms with animals showing signs of enteritis; those samples were resulting negative for Mycobacterium avium subsp. paratuberculosis (MAP) IS900 PCR but IS1612 presence in the samples, represented by stool and gut tracts, was demonstrated by real time PCR and capillary sequencing methods. The real time PCR detected in reconstructed samples about 100 CFU/g and showed a high linear dynamic range of quantification (102-106 IS1612 copies DNA/reaction) with a good correlation rate (R2 = 0.98). Results suggest that real time PCR assay represents a rapid and accurate molecular method to detect/quantify Mycobacterium avium subsp. silvaticum in biological samples.
Rilevamento di Mycobacterium avium subsp. silvaticum in campioni clinici di ovino mediante PCR real time
ORRU, GERMANO;ISOLA, DANIELA;
2007-01-01
Abstract
SUMMARY We have identified and quantified a Mycobacterium avium subsp. silvaticum (MAS) 1612 insertion sequence in ovine clinical samples, derived from two different sheep farms with animals showing signs of enteritis; those samples were resulting negative for Mycobacterium avium subsp. paratuberculosis (MAP) IS900 PCR but IS1612 presence in the samples, represented by stool and gut tracts, was demonstrated by real time PCR and capillary sequencing methods. The real time PCR detected in reconstructed samples about 100 CFU/g and showed a high linear dynamic range of quantification (102-106 IS1612 copies DNA/reaction) with a good correlation rate (R2 = 0.98). Results suggest that real time PCR assay represents a rapid and accurate molecular method to detect/quantify Mycobacterium avium subsp. silvaticum in biological samples.
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