Genetic characterization and artificial reproduction attempt of endemic Sardinian trout Salmo trutta L., 1758 (Osteichthyes, Salmonidae): Experiences in captivity

Native species of Sardinian brown trout (Salmo trutta) have declined drastically because of the introduction of non-native salmonids, overharvesting, and habitat degradation. One approach to conservation of declining species is to establish new populations through repopulation with genetically certified fish. In this study, samples of brown trout from seven rivers in Sardinia were analysed to look for the presence of pure individuals of the endemic Sardinian trout. Analyses of the mitochondrial DNA control region and the LDH-C1* gene revealed the presence of non-endemic and hybrid individuals in four of the seven rivers analysed. In addition, we reared a stock of native Sardinian trout at the experimental fish farm of Sadali and had them reproduce artificially. This experiment provided more knowledge about the incubation phase, hatching, reabsorption of the vitelline sac and the initial phases of growth, which can be used to develop more optimal management of this resource