In line with the Self-Determination Theory (SDT), the scale of motivation at work (MAWS) was developed through a multi-dimensional approach and validated in English and French language. This work examined the structure of the MAWS in Italian organizational context. By using two samples (Study 1, N = 525; Study 2, N = 465), the results of exploratory factor analysis (Study 1) suggested a structure of work motivation organized in three factors: autonomous, introjected and external motivation, confirmed by a confirmatory (Study 2). The convergent and discriminant validity of the subscales was assessed. We discussed the importance of this scale for the development of future research based on SDT in the organizational context.