Formazione di Biofilm da Enterococcus faecalis in diversi preparati endodontici: valutazione mediante metodi molecolari

Objective: The adhesion abilities of Enterococcus Faecalis in the root canals represent the essential condition during endodontic pathologies, because of the biofilm nature of these diseases. In this contest, the present study investigated the adhesion of E Faecalis in 3 different endodontic medicaments by a biofilm model in vitro of E Faecalis: (i) CPD, (ii) Endoidrox, (iii) PulpCanalSealer. Methods: The adhesion index of the samples was evaluated for 10 days after shaking the cultures at 37°C, by using quantitative real-time PCR for the 16S rRNA gene. Results: The highest concentration of adherent bacteria was found after 9 days in the PulpCanalSealer (maximum level of the adhesion index = 2.9x104), followed by CPD (2.7x103), while Endoidrox showed the lowest adhesion index (5x102). None of these intracanalar preparations showed antibacterial activity against E Faecalis. Conclusions: The present study confirmed the E Faecalis ability to persist in any endodontic medicament. In addition our work evaluated the adhesion index of E Faecalis biofilm in three different medicament preparations. Our results may contribute to the explanation of how the microorganism can survive inside filled chambers.