The present study is focused on the antioxidant capacity and chemical profiling of eight Croatian Satureja Montana L. honey samples. Among the 20 compounds obtained by headspace solid-phase microextraction (HS-SPME) and identified by GC-FID and GC/MS analyses, hotrienol was predominant (75.9-81.7%). The honey matrix volatile/semivolatile profile was investigated by ultrasonic solvent extraction (USE) followed by GC-FID and GC/MS analyses. The major compounds identified by this latter method were the sinapic-acid derivatives methyl syringate (36.2-72.8%) and syringaldehyde (2.2-43.1%). Direct, targeted HPLC-DAD analyses of the native honey samples revealed the presence of methyl syringate (7.10-39.60 mg/kg) and syringic acid (0.10-1.70 mg/kg). In addition, the total phenolic content of the samples was determined by the Folin£Ciocalteu assay (311.0-465.9 mg GAE/kg), and the antioxidant capacity was evaluated by the DPPH radical-scavenging activity (0.5-1.0 mmol TEAC/kg) and the ferric reducing antioxidant power (2.5-5.1 mmol Fe2+/kg)

Antioxidant capacity and chemical profiles of Satureja Montana L. Honey: hotrienol and syringyl derivatives as biomarkers

TUBEROSO, CARLO IGNAZIO GIOVANNI;
2015

Abstract

The present study is focused on the antioxidant capacity and chemical profiling of eight Croatian Satureja Montana L. honey samples. Among the 20 compounds obtained by headspace solid-phase microextraction (HS-SPME) and identified by GC-FID and GC/MS analyses, hotrienol was predominant (75.9-81.7%). The honey matrix volatile/semivolatile profile was investigated by ultrasonic solvent extraction (USE) followed by GC-FID and GC/MS analyses. The major compounds identified by this latter method were the sinapic-acid derivatives methyl syringate (36.2-72.8%) and syringaldehyde (2.2-43.1%). Direct, targeted HPLC-DAD analyses of the native honey samples revealed the presence of methyl syringate (7.10-39.60 mg/kg) and syringic acid (0.10-1.70 mg/kg). In addition, the total phenolic content of the samples was determined by the Folin£Ciocalteu assay (311.0-465.9 mg GAE/kg), and the antioxidant capacity was evaluated by the DPPH radical-scavenging activity (0.5-1.0 mmol TEAC/kg) and the ferric reducing antioxidant power (2.5-5.1 mmol Fe2+/kg)
Antioxidant activity, GC/MS analysis, Headspace solid-phase microextraction (HS-SPME), Honey, Hotrienol, HPLC-DAD analysis, Methyl syringate, Satureja Montana, Syringaldehyde, Syringic acid, Ultrasonic solvent extraction (USE), Alkenes, Antioxidants, Benzaldehydes, Biomarkers, Coumaric acids, Gallic acid, Gas chromatography-mass spectrometry, Satureja, Solid phase microextraction, Honey, Biochemistry, Chemistry (all), Bioengineering, Molecular biology, Molecular medicine
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11584/173939
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