A high-performance liquid chromatographic method is reported that allows the determination of organic acids in honey after sample purification by solid-phase extraction. The chromatographic separation was achieved with two Spherisorb ODS-1 S5 columns connected in series and sulphuric acid (pH 2.45) as the mobile phase. The average recoveries of the acids ranged from 89% to 104% and the detection limits from 0.002 to 3 ppm (w/w).
Solid-phase extraction and high-performance liquid chromatographic determination of organic acids in honey
TUBEROSO, CARLO IGNAZIO GIOVANNI;
1994-01-01
Abstract
A high-performance liquid chromatographic method is reported that allows the determination of organic acids in honey after sample purification by solid-phase extraction. The chromatographic separation was achieved with two Spherisorb ODS-1 S5 columns connected in series and sulphuric acid (pH 2.45) as the mobile phase. The average recoveries of the acids ranged from 89% to 104% and the detection limits from 0.002 to 3 ppm (w/w).
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