Coffea spp. honey was screened by UV/VIS, HS-SPME/GC-MS/FID, USE/GC-MS/FID and HPLC-DAD. The direct HPLC-DAD methodology overcame the major limitations of the other methods used. The obtained results constitute a breakthrough since dominant xanthine derivatives were found (theobromine and caffeine with HPLC-DAD and caffeine by USE/GC-MS/FID). Phenylacetaldehyde was the major headspace compound.
Screening of Coffea spp. honey by different methodologies: theobromine and caffeine as chemical markers
TUBEROSO, CARLO IGNAZIO GIOVANNI;
2014-01-01
Abstract
Coffea spp. honey was screened by UV/VIS, HS-SPME/GC-MS/FID, USE/GC-MS/FID and HPLC-DAD. The direct HPLC-DAD methodology overcame the major limitations of the other methods used. The obtained results constitute a breakthrough since dominant xanthine derivatives were found (theobromine and caffeine with HPLC-DAD and caffeine by USE/GC-MS/FID). Phenylacetaldehyde was the major headspace compound.
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