For the first time we report a rapid and sensitive LC-MS-MS method for quantification of rotenone, deguelin, and rotenolone in human serum. The analytical procedure involves extraction with ethyl acetate without further clean-up. The active ingredients were separated on a C 8 reversed-phase column by isocratic elution. Eleven simultaneous transitions of precursor ions were monitored. Excellent selectivity and sensitivity enables quantification and identification of low levels of rotenoids (LOD 2 ng mL -1, LOQ 5 ng mL -1) in human serum.

LC-MS-MS determination of rotenone, deguelin, and rotenolone in human serum

CABONI, PIERLUIGI;SARAIS, GIORGIA;VARGIU, SIMONA;DE LUCA, MARIA ANTONIETTA;GARAU, VINCENZO;IBBA, ANTONIO;
2008

Abstract

For the first time we report a rapid and sensitive LC-MS-MS method for quantification of rotenone, deguelin, and rotenolone in human serum. The analytical procedure involves extraction with ethyl acetate without further clean-up. The active ingredients were separated on a C 8 reversed-phase column by isocratic elution. Eleven simultaneous transitions of precursor ions were monitored. Excellent selectivity and sensitivity enables quantification and identification of low levels of rotenoids (LOD 2 ng mL -1, LOQ 5 ng mL -1) in human serum.
Column liquid chromatography-tandem mass spectrometry; Deguelin; Human serum; Rotenolone; Rotenone
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/106766
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