Isolation of essential oil from dried leaves of Juniperus phoenicea of Tunisia were obtained with two different methods: by means of the classical hydrodistillation (HD) and by using carbon dioxide in the supercritical state (SFE). Operative conditions adopted in SFE were: pressure, 90 bar and temperature, 50 °C. The leaf volatile oil was made up of germacrene D (12.6 %), (E)-caryophyllene (7.2 %), cubebol (6.5 %), ô-cadinene (6.0 %) and Ot-terpinyl acetate (5.6 %). The corresponding yield (w/w) was 0.64 %. The essential oil obtained by hydrodistillation at a yield of (0.26 %) was characterized by Ot-pinene (16.4 %), ô-cadinene (8.1 %) germacrene D (8.0 %), a-terpinyl acetate (7.1 %) and (E)-caryophyllene (5.8 %) as main constituents. These oils failed to inhibit three microorganisms tested (Pseudomonas aeruginosa, Escherchia coli and Enterococcus faecalis) at the highest concentration tested (MIC > 10 %), but they exhibited a very good activity against Candida albicans at MIC 0.0125 %. Moreover, both hydrodistilled and supercritical extracted oils were found to inhibit Staphylococcus aureus growth, in special way the former was more active at concentration of 0.6 % v/v. © 2008 Taylor & Francis Group, LLC.

Chemical characterization and evaluation of biological activity of essential oil of Juniperus phoenicea of Tunisia

PIRAS, ALESSANDRA;PORCEDDA, SILVIA;TUVERI, ENRICA
2008-01-01

Abstract

Isolation of essential oil from dried leaves of Juniperus phoenicea of Tunisia were obtained with two different methods: by means of the classical hydrodistillation (HD) and by using carbon dioxide in the supercritical state (SFE). Operative conditions adopted in SFE were: pressure, 90 bar and temperature, 50 °C. The leaf volatile oil was made up of germacrene D (12.6 %), (E)-caryophyllene (7.2 %), cubebol (6.5 %), ô-cadinene (6.0 %) and Ot-terpinyl acetate (5.6 %). The corresponding yield (w/w) was 0.64 %. The essential oil obtained by hydrodistillation at a yield of (0.26 %) was characterized by Ot-pinene (16.4 %), ô-cadinene (8.1 %) germacrene D (8.0 %), a-terpinyl acetate (7.1 %) and (E)-caryophyllene (5.8 %) as main constituents. These oils failed to inhibit three microorganisms tested (Pseudomonas aeruginosa, Escherchia coli and Enterococcus faecalis) at the highest concentration tested (MIC > 10 %), but they exhibited a very good activity against Candida albicans at MIC 0.0125 %. Moreover, both hydrodistilled and supercritical extracted oils were found to inhibit Staphylococcus aureus growth, in special way the former was more active at concentration of 0.6 % v/v. © 2008 Taylor & Francis Group, LLC.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11584/99879
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